› 6o3b

X-ray diffraction
2.5Å resolution

Crystal structure of Frizzled 7 CRD in complex with F6 Fab

Released:
DOI: 10.2210/pdb6o3b/pdb
PDB entry 6o3b coloured by chain, front view.
PDB entry 6o3b coloured by chain, side view.
PDB entry 6o3b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-guided design fine-tunes pharmacokinetics, tolerability, and antitumor profile of multispecific frizzled antibodies.
Raman S, Beilschmidt M, To M, Lin K, Lui F, Jmeian Y, Ng M, Fernandez M, Fu Y, Mascall K, Duque A, Wang X, Pan G, Angers S, Moffat J, Sidhu SS, Magram J, Sinclair AM, Fransson J, Julien JP
Proc Natl Acad Sci U S A 116 6812-6817 (2019)
PMID: 30894493

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231674 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody Fab F6, Light chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 214 amino acids
Theoretical weight: 23.36 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Antibody Fab F6, Heavy chain Chains: B, G
Molecule details ›
Chains: B, G
Length: 221 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Frizzled-7 Chains: C, H
Molecule details ›
Chains: C, H
Length: 140 amino acids
Theoretical weight: 15.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O75084 (Residues: 42-179; Coverage: 26%)
Gene name: FZD7
Sequence domains: Fz domain
Structure domains: Frizzled cysteine-rich domain

Ligands and Environments

2 bound ligands:
Ligand GOL 5 x GOL
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 139.642Å b: 85.848Å c: 104.103Å
α: 90° β: 95.82° γ: 90°
R-values:
R R work R free
0.25 0.248 0.283
Expression system: Homo sapiens

Quick links

Citations

5 review citations

Aberrant WNT/CTNNB1 Signaling as a Therapeutic Target in Human Breast Cancer: Weighing the Evidence.
van Schie et al. (2020)
4 more

1 mention without citation

Structure-guided design fine-tunes pharmacokinetics, tolerability, and antitumor profile of multispecific frizzled antibodies.
Raman et al. (2019)