› 6ob1

Solution NMR

Structure of WHB in complex with Ubiquitin Variant

Released:
DOI: 10.2210/pdb6ob1/pdb
PDB entry 6ob1 coloured by chain, ensemble of 20 models, front view.
PDB entry 6ob1 coloured by chain, ensemble of 20 models, side view.
PDB entry 6ob1 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Protein engineering of a ubiquitin-variant inhibitor of APC/C identifies a cryptic K48 ubiquitin chain binding site.
Watson ER, Grace CRR, Zhang W, Miller DJ, Davidson IF, Prabu JR, Yu S, Bolhuis DL, Kulko ET, Vollrath R, Haselbach D, Stark H, Peters JM, Brown NG, Sidhu SS, Schulman BA
Proc Natl Acad Sci U S A 116 17280-17289 (2019)
PMID: 31350353

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167192 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 75 amino acids
Theoretical weight: 8.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 75 amino acids
Theoretical weight: 8.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
Anaphase-promoting complex subunit 2 Chain: C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 67%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

Quick links

Citations

4 review citations

Ubiquitin ligases: guardians of mammalian development.
Cruz Walma et al. (2022)
3 more

2 mentions without citation

Ubiquitin Engineering for Interrogating the Ubiquitin-Proteasome System and Novel Therapeutic Strategies.
Tang et al. (2023)
1 more

Experimental raw data

Links to raw experimental data available for this entry are listed below

NMR data related to PDB entry 6ob1 available at Biological Magnetic Resonance Data Bank (BMRB)

BMRB accession: BMR30590

Available data:
  • Assigned chemical shifts: 1
  • Spectral peak lists: 1