› 6oph

X-ray diffraction
2.4Å resolution

phosphorylated ERK2 with GDC-0994

Released:
DOI: 10.2210/pdb6oph/pdb
PDB entry 6oph coloured by chain, front view.
PDB entry 6oph coloured by chain, side view.
PDB entry 6oph coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Activation loop dynamics are controlled by conformation-selective inhibitors of ERK2.
Pegram LM, Liddle JC, Xiao Y, Hoh M, Rudolph J, Iverson DB, Vigers GP, Smith D, Zhang H, Wang W, Moffat JG, Ahn NG
Proc Natl Acad Sci U S A 116 15463-15468 (2019)
PMID: 31311868

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151230 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 41.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P28482 (Residues: 8-360; Coverage: 98%)
Gene names: ERK2, MAPK1, PRKM1, PRKM2
Sequence domains: Protein kinase domain

Ligands and Environments

1 bound ligand:
Ligand 6QB 1 x 6QB
2 modified residues:
Ligand TPO 1 x TPO
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: P212121
Unit cell:
a: 42.07Å b: 76.93Å c: 151.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.243
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Mutations That Confer Drug-Resistance, Oncogenicity and Intrinsic Activity on the ERK MAP Kinases-Current State of the Art.
Smorodinsky-Atias et al. (2020)
1 more

10 mentions without citation

Activation loop dynamics are controlled by conformation-selective inhibitors of ERK2.
Pegram et al. (2019)
9 more