› 6owt

Electron Microscopy
3.8Å resolution

Structure of SIVsmm Nef and SMM tetherin bound to the clathrin adaptor AP-2 complex

Released:
DOI: 10.2210/pdb6owt/pdb
PDB entry 6owt coloured by chain, front view.
PDB entry 6owt coloured by chain, side view.
PDB entry 6owt coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for Tetherin Antagonism as a Barrier to Zoonotic Lentiviral Transmission.
Buffalo CZ, Stürzel CM, Heusinger E, Kmiec D, Kirchhoff F, Hurley JH, Ren X
Cell Host Microbe 26 359-368.e8 (2019)
PMID: 31447307
Related structures: EMD-20217

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-111231 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
AP-2 complex subunit alpha-2 Chain: A
Molecule details ›
Chain: A
Length: 939 amino acids
Theoretical weight: 104.29 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18484 (Residues: 1-271, 272-938; Coverage: 100%)
Gene names: Adtab, Ap2a2
Sequence domains:
AP-2 complex subunit beta Chain: B
Molecule details ›
Chain: B
Length: 591 amino acids
Theoretical weight: 66.95 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P62944 (Residues: 1-591; Coverage: 63%)
Gene names: Ap2b1, Clapb1
Sequence domains: Adaptin N terminal region
AP-2 complex subunit mu Chain: M
Protein Nef; Tetherin variant Sm2 Chains: N, T
AP-2 complex subunit sigma Chain: S

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.8Å
Relevant EMDB volumes: EMD-20217
Expression system: Escherichia coli

Quick links

Citations

4 review citations

A tug-of-war between severe acute respiratory syndrome coronavirus 2 and host antiviral defence: lessons from other pathogenic viruses.
Fung et al. (2020)
3 more

3 mentions without citation

How HIV Nef Proteins Hijack Membrane Traffic To Promote Infection.
Buffalo et al. (2019)
2 more