6owt

Electron Microscopy
3.8Å resolution

Structure of SIVsmm Nef and SMM tetherin bound to the clathrin adaptor AP-2 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-111231 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
AP-2 complex subunit alpha-2 Chain: A
Molecule details ›
Chain: A
Length: 939 amino acids
Theoretical weight: 104.29 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18484 (Residues: 1-271, 272-938; Coverage: 100%)
Gene names: Adtab, Ap2a2
Sequence domains:
AP-2 complex subunit beta Chain: B
Molecule details ›
Chain: B
Length: 591 amino acids
Theoretical weight: 66.95 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P62944 (Residues: 1-591; Coverage: 63%)
Gene names: Ap2b1, Clapb1
Sequence domains: Adaptin N terminal region
AP-2 complex subunit mu Chain: M
Molecule details ›
Chain: M
Length: 141 amino acids
Theoretical weight: 16.16 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P84091 (Residues: 1-141; Coverage: 32%)
Gene names: Ap2m1, Clapm1
Sequence domains: Clathrin adaptor complex small chain
Protein Nef; Tetherin variant Sm2 Chains: N, T
Molecule details ›
Chains: N, T
Length: 275 amino acids
Theoretical weight: 31.77 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: C3VHQ5 (Residues: 4-24, 87-88; Coverage: 13%)
  • Canonical: Q4JGV0 (Residues: 1-54, 57-251; Coverage: 91%)
Gene names: BST2, nef
Sequence domains: Negative factor, (F-Protein) or Nef
AP-2 complex subunit sigma Chain: S
Molecule details ›
Chain: S
Length: 142 amino acids
Theoretical weight: 17.01 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P62744 (Residues: 1-142; Coverage: 100%)
Gene names: Ap17, Ap2s1, Claps2
Sequence domains: Clathrin adaptor complex small chain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-20217
Expression system: Escherichia coli