› 6p8c

X-ray diffraction
2.07Å resolution

2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase (MthRED) from Methanothermobacter thermautotrophicus

Released:
DOI: 10.2210/pdb6p8c/pdb
Model geometry
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PDB entry 6p8c coloured by chain, front view.
PDB entry 6p8c coloured by chain, side view.
PDB entry 6p8c coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Entry authors: Carbone V, Schofield LR, Hannus I, Sutherland-Smith AJ, Ronimus RS

Function and Biology Details

Reaction catalysed: 
2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NAD(P)(+) = 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 27.4 KDa
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Expression system: Escherichia coli
UniProt:
  • Canonical: O26337 (Residues: 1-216; Coverage: 100%)
Gene name: MTH_235
Sequence domains: RibD C-terminal domain

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
3 bound ligands:
Ligand CL 2 x CL
Ligand GOL 4 x GOL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 56.921Å b: 89.597Å c: 107.013Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.222
Expression system: Escherichia coli

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