› 6p8s

X-ray diffraction
2Å resolution

Structure of P. aeruginosa ATCC27853 HORMA1:HORMA2:Peptide 1 complex

Released:
DOI: 10.2210/pdb6p8s/pdb
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PDB entry 6p8s coloured by chain, front view.
PDB entry 6p8s coloured by chain, side view.
PDB entry 6p8s coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
HORMA Domain Proteins and a Trip13-like ATPase Regulate Bacterial cGAS-like Enzymes to Mediate Bacteriophage Immunity.
Ye Q, Lau RK, Mathews IT, Birkholz EA, Watrous JD, Azimi CS, Pogliano J, Jain M, Corbett KD
Mol Cell 77 709-722.e7 (2020)
PMID: 31932165

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-145115 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
CD-NTase-associated protein 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 18.72 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DTF6 (Residues: 1-166; Coverage: 100%)
Gene names: A4W92_29530, HORMA2, cap7
Sequence domains: Bacterial HORMA domain 2
CD-NTase-associated protein 8 Chains: C, D
Molecule details ›
Chains: C, D
Length: 133 amino acids
Theoretical weight: 14.41 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DTF5 (Residues: 1-133; Coverage: 85%)
Gene names: A4W92_29525, HORMA3, cap8
Peptide 1 Chains: E, F

Ligands and Environments

3 bound ligands:
Ligand ACT 2 x ACT
Ligand CL 3 x CL
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2221
Unit cell:
a: 100.892Å b: 124.539Å c: 109.141Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.227
Expression system: Escherichia coli

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Citations

18 review citations

Conserved strategies for pathogen evasion of cGAS-STING immunity.
Eaglesham et al. (2020)
17 more

1 mention without citation

HORMA Domain Proteins and a Trip13-like ATPase Regulate Bacterial cGAS-like Enzymes to Mediate Bacteriophage Immunity.
Ye et al. (2020)

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