› 6pcv

Electron Microscopy
3.2Å resolution

Single Particle Reconstruction of Phosphatidylinositol (3,4,5) trisphosphate-dependent Rac exchanger 1 bound to G protein beta gamma subunits

Released:
DOI: 10.2210/pdb6pcv/pdb
PDB entry 6pcv coloured by chain, front view.
PDB entry 6pcv coloured by chain, side view.
PDB entry 6pcv coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-electron microscopy structure and analysis of the P-Rex1-Gβγ signaling scaffold.
Cash JN, Urata S, Li S, Ravala SK, Avramova LV, Shost MD, Gutkind JS, Tesmer JJG, Cianfrocco MA
OpenAccess logo Sci Adv 5 eaax8855 (2019)
PMID: 31663027
Related structures: EMD-20308

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-158740 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein Chain: A
Molecule details ›
Chain: A
Length: 1635 amino acids
Theoretical weight: 184.84 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8TCU6 (Residues: 38-1659; Coverage: 98%)
Gene names: KIAA1415, PREX1
Sequence domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62871 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 81 amino acids
Theoretical weight: 9.23 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-20308
Expression systems:
  • Homo sapiens
  • Trichoplusia ni

Quick links

Citations

4 review citations

Complementarity of Hydrogen/Deuterium Exchange Mass Spectrometry and Cryo-Electron Microscopy.
Engen et al. (2020)
3 more

3 mentions without citation

The first DEP domain of the RhoGEF P-Rex1 autoinhibits activity and contributes to membrane binding.
Ravala et al. (2020)
2 more