› 6pze

X-ray diffraction
2.3Å resolution

Crystal structure of human NA-45 Fab in complex with neuraminidase Y169aH mutant from A/Shanghai/2/2013 (H7N9)

Released:
DOI: 10.2210/pdb6pze/pdb
PDB entry 6pze coloured by chain, front view.
PDB entry 6pze coloured by chain, side view.
PDB entry 6pze coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis of Protection against H7N9 Influenza Virus by Human Anti-N9 Neuraminidase Antibodies.
Zhu X, Turner HL, Lang S, McBride R, Bangaru S, Gilchuk IM, Yu W, Paulson JC, Crowe JE, Ward AB, Wilson IA
Cell Host Microbe 26 729-738.e4 (2019)
PMID: 31757767

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dodecamer
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232188 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Neuraminidase Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 44.01 KDa
Source organism: Influenza A virus (A/Shanghai/02/2013(H7N9))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: R4NFR6 (Residues: 78-465; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
NA-45 FAB LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 22.91 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
NA-45 FAB HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 237 amino acids
Theoretical weight: 25.36 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P4212
Unit cell:
a: 161.268Å b: 161.268Å c: 87.382Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.197
Expression systems:
  • Spodoptera frugiperda
  • Cricetulus griseus

Quick links

Citations

9 review citations

Innovations in structure-based antigen design and immune monitoring for next generation vaccines.
Ward et al. (2020)
8 more

3 mentions without citation

Broadly Protective Neuraminidase-Based Influenza Vaccines and Monoclonal Antibodies: Target Epitopes and Mechanisms of Action.
Abbadi et al. (2023)
2 more