› 6q1z

X-ray diffraction
3.45Å resolution

Crystal structure of human 1G04 Fab in complex with influenza virus neuraminidase from A/Hunan/02650/2016 (H7N9)

Released:
DOI: 10.2210/pdb6q1z/pdb
PDB entry 6q1z coloured by chain, front view.
PDB entry 6q1z coloured by chain, side view.
PDB entry 6q1z coloured by chain, top view.
Source organisms:
Primary publication:
Broadly protective human antibodies that target the active site of influenza virus neuraminidase.
Stadlbauer D, Zhu X, McMahon M, Turner JS, Wohlbold TJ, Schmitz AJ, Strohmeier S, Yu W, Nachbagauer R, Mudd PA, Wilson IA, Ellebedy AH, Krammer F
Science 366 499-504 (2019)
PMID: 31649200

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231569 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Neuraminidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 43.98 KDa
Source organism: Influenza A virus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A5A4LHS4 (Residues: 76-465; Coverage: 84%)
Gene name: NA
Sequence domains: Neuraminidase
1G04 Fab kappa light chain Chains: C, L
Molecule details ›
Chains: C, L
Length: 216 amino acids
Theoretical weight: 23.56 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
1G04 Fab IgG1 heavy chain Chains: D, H
Molecule details ›
Chains: D, H
Length: 240 amino acids
Theoretical weight: 26.11 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I4
Unit cell:
a: 180.242Å b: 180.242Å c: 148.125Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.247
Expression systems:
  • Spodoptera frugiperda
  • Cricetulus griseus

Quick links

Citations

32 review citations

Next-generation influenza vaccines: opportunities and challenges.
Wei et al. (2020)
31 more

3 mentions without citation

The Human Antibody Response to the Influenza Virus Neuraminidase Following Infection or Vaccination.
Rajendran et al. (2021)
2 more