› 6qaf

X-ray diffraction
1.61Å resolution

Crystal structure of human Arginase-1 at pH 9.0 in complex with CB-1158/INCB001158

Released:
DOI: 10.2210/pdb6qaf/pdb
PDB entry 6qaf coloured by chain, front view.
PDB entry 6qaf coloured by chain, side view.
PDB entry 6qaf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into human Arginase-1 pH dependence and its inhibition by the small molecule inhibitor CB-1158.
Grobben Y, Uitdehaag JCM, Willemsen-Seegers N, Tabak WWA, de Man J, Buijsman RC, Zaman GJR
OpenAccess logo J Struct Biol X 4 100014 (2020)
PMID: 32647818

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-ornithine + urea
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-529026 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginase-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 36.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05089 (Residues: 1-322; Coverage: 100%)
Gene name: ARG1
Sequence domains: Arginase family

Ligands and Environments

3 bound ligands:
Ligand MN 4 x MN
Ligand XC3 2 x XC3
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P3
Unit cell:
a: 90.13Å b: 90.13Å c: 69.111Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.147 0.177
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Arginase as a Potential Biomarker of Disease Progression: A Molecular Imaging Perspective.
S Clemente et al. (2020)
2 more

2 mentions without citation

Allosteric Coupling of Drug Binding and Intracellular Signaling in the A2A Adenosine Receptor.
Eddy et al. (2018)
1 more