› 6qi6

X-ray diffraction
2Å resolution

Trigonal form of WT recombinant bovine beta-lactoglobulin

Released:
DOI: 10.2210/pdb6qi6/pdb
PDB entry 6qi6 coloured by chain, front view.
PDB entry 6qi6 coloured by chain, side view.
PDB entry 6qi6 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure-based design approach to rational site-directed mutagenesis of β-lactoglobulin.
Bonarek P, Loch JI, Tworzydło M, Cooper DR, Milto K, Wróbel P, Kurpiewska K, Lewiński K
J Struct Biol 210 107493 (2020)
PMID: 32169624

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactoglobulin Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.23 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02754 (Residues: 17-178; Coverage: 100%)
Gene name: LGB
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand EDO 2 x EDO
Ligand EOH 1 x EOH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Agilent SuperNova
Spacegroup: P3221
Unit cell:
a: 52.732Å b: 52.732Å c: 110.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.186 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Milk Processing Affects Structure, Bioavailability and Immunogenicity of β-lactoglobulin.
Broersen K. (2020)
3 other articles

2 mentions without citation

Simulation of Energy-Resolved Mass Spectrometry Distributions from Surface-Induced Dissociation.
Seffernick et al. (2022)
1 more