› 6qs1

X-ray diffraction
1.8Å resolution

Crystal structure of human Angiotensin-1 converting enzyme N-domain in complex with BPPb

Released:
DOI: 10.2210/pdb6qs1/pdb
PDB entry 6qs1 coloured by chain, front view.
PDB entry 6qs1 coloured by chain, side view.
PDB entry 6qs1 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the C-domain-selective angiotensin-converting enzyme inhibition by bradykinin-potentiating peptide b (BPPb).
Sturrock ED, Lubbe L, Cozier GE, Schwager SLU, Arowolo AT, Arendse LB, Belcher E, Acharya KR
Biochem J 476 1553-1570 (2019)
PMID: 31072910

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-537002 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (6 distinct):
Angiotensin-converting enzyme, soluble form Chains: A, B
Molecule details ›
Chains: A, B
Length: 629 amino acids
Theoretical weight: 72.61 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P12821 (Residues: 30-657; Coverage: 49%)
Gene names: ACE, DCP, DCP1
Sequence domains: Angiotensin-converting enzyme
Bradykinin potentiating peptide b Chains: E, F
Molecule details ›
Chains: E, F
Length: 11 amino acids
Theoretical weight: 1.18 KDa
Source organism: Gloydius blomhoffii
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
9 bound ligands:
Ligand XPE 1 x XPE
Ligand EDO 6 x EDO
Ligand PEG 4 x PEG
Ligand BO3 7 x BO3
Ligand PGE 1 x PGE
Ligand ZN 2 x ZN
Ligand CL 4 x CL
Ligand MG 2 x MG
Ligand NAG 1 x NAG
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 72.682Å b: 77.07Å c: 82.363Å
α: 88.86° β: 64.59° γ: 75.03°
R-values:
R R work R free
0.202 0.202 0.232
Expression systems:
  • Cricetulus griseus
  • Not provided

Quick links

Citations

2 review citations

Novel Therapeutic Approaches Targeting the Renin-Angiotensin System and Associated Peptides in Hypertension and Heart Failure.
Arendse et al. (2019)
1 more

3 mentions without citation

The chemistry of snake venom and its medicinal potential.
Oliveira et al. (2022)
2 more