› 6r79

X-ray diffraction
1.9Å resolution

Structure of IMP-13 metallo-beta-lactamase in apo form (loop open)

Released:
DOI: 10.2210/pdb6r79/pdb
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PDB entry 6r79 coloured by chain, front view.
PDB entry 6r79 coloured by chain, side view.
PDB entry 6r79 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure and Molecular Recognition Mechanism of IMP-13 Metallo-β-Lactamase.
Softley CA, Zak KM, Bostock MJ, Fino R, Zhou RX, Kolonko M, Mejdi-Nitiu R, Meyer H, Sattler M, Popowicz GM
OpenAccess logo Antimicrob Agents Chemother 64 (2020)
PMID: 32205343

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182156 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 220 amino acids
Theoretical weight: 24.57 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7WYA8 (Residues: 22-241; Coverage: 97%)
Gene names: bla-imp13, blaIMP

Ligands and Environments

6 bound ligands:
Ligand GOL 6 x GOL
Ligand BME 6 x BME
Ligand GLY 13 x GLY
Ligand ZN 8 x ZN
Ligand NA 1 x NA
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P1
Unit cell:
a: 44.762Å b: 64.825Å c: 86.546Å
α: 107.52° β: 90.99° γ: 94.8°
R-values:
R R work R free
0.177 0.175 0.214
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

Different Conformations Revealed by NMR Underlie Resistance to Ceftazidime/Avibactam and Susceptibility to Meropenem and Imipenem among D179Y Variants of KPC β-Lactamase.
Taracila et al. (2022)
1 more

3 mentions without citation

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.
Bahr et al. (2021)
2 more

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