› 6rk0

X-ray diffraction
0.99Å resolution

Structure of the Flavocytochrome Anf3 from Azotobacter vinelandii

Released:
DOI: 10.2210/pdb6rk0/pdb
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PDB entry 6rk0 coloured by chain, front view.
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PDB entry 6rk0 coloured by chain, top view.
Source organism: Azotobacter vinelandii DJ
Primary publication:
A low-potential terminal oxidase associated with the iron-only nitrogenase from the nitrogen-fixing bacterium Azotobacter vinelandii.
Varghese F, Kabasakal BV, Cotton CAR, Schumacher J, Rutherford AW, Fantuzzi A, Murray JW
OpenAccess logo J Biol Chem 294 9367-9376 (2019)
PMID: 31043481

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111094 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin-nucleotide-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 25.4 KDa
Source organism: Azotobacter vinelandii DJ
Expression system: Escherichia coli KRX
UniProt:
  • Canonical: C1DK98 (Residues: 1-223; Coverage: 100%)
Gene name: Avin_49040
Sequence domains: Pyridoxamine 5'-phosphate oxidase

Ligands and Environments

3 bound ligands:
Ligand HEM 2 x HEM
Ligand SO4 2 x SO4
Ligand FAD 2 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 48.97Å b: 89.87Å c: 59.03Å
α: 90° β: 109.06° γ: 90°
R-values:
R R work R free
0.124 0.124 0.138
Expression system: Escherichia coli KRX

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Citations

3 review citations

Multi-Therapeutic Potential of Naringenin (4',5,7-Trihydroxyflavonone): Experimental Evidence and Mechanisms.
Arafah et al. (2020)
2 more

3 mentions without citation

A low-potential terminal oxidase associated with the iron-only nitrogenase from the nitrogen-fixing bacterium Azotobacter vinelandii.
Varghese et al. (2019)
2 more

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