› 6ry5

X-ray diffraction
1.3Å resolution

Crystal structure of Dfg5 from Chaetomium thermophilum in complex with alpha-1,6-mannobiose

Released:
DOI: 10.2210/pdb6ry5/pdb
PDB entry 6ry5 coloured by chain, front view.
PDB entry 6ry5 coloured by chain, side view.
PDB entry 6ry5 coloured by chain, top view.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
Structural base for the transfer of GPI-anchored glycoproteins into fungal cell walls.
Vogt MS, Schmitz GF, Varón Silva D, Mösch HU, Essen LO
Proc Natl Acad Sci U S A 117 22061-22067 (2020)
PMID: 32839341

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123980 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Mannan endo-1,6-alpha-mannosidase Chain: A
Molecule details ›
Chain: A
Length: 443 amino acids
Theoretical weight: 49.55 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli B
UniProt:
  • Canonical: G0S3F2 (Residues: 30-449; Coverage: 94%)
Gene name: CTHT_0020800
Sequence domains: Glycosyl hydrolase family 76

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand CA 2 x CA
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 83.415Å b: 55.019Å c: 80.119Å
α: 90° β: 90.37° γ: 90°
R-values:
R R work R free
0.103 0.102 0.123
Expression system: Escherichia coli B

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Citations

1 review citation

FLO11, a Developmental Gene Conferring Impressive Adaptive Plasticity to the Yeast Saccharomyces cerevisiae.
Bouyx et al. (2021)
14 other articles