› 6s0i

X-ray diffraction
1.8Å resolution

Crystal structure of Escherichia coli Glyoxalase II with L-Tartrate in the active site

Released:
DOI: 10.2210/pdb6s0i/pdb
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PDB entry 6s0i coloured by chain, front view.
PDB entry 6s0i coloured by chain, side view.
PDB entry 6s0i coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Skorupskaite A, McDonough MA, Schofield CJ

Function and Biology Details

Reaction catalysed: 
S-(2-hydroxyacyl)glutathione + H(2)O = glutathione + a 2-hydroxy carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydroxyacylglutathione hydrolase GloB Chain: A
Molecule details ›
Chain: A
Length: 251 amino acids
Theoretical weight: 28.47 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AC84 (Residues: 1-251; Coverage: 100%)
Gene names: JW0202, b0212, gloB, yafR
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand TLA 1 x TLA
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 39.053Å b: 43.655Å c: 159.067Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.161 0.197
Expression system: Escherichia coli BL21(DE3)

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