› 6s25

X-ray diffraction
1.1Å resolution

Crystal Structure of the first bromodomain of BRD4 in complex with a benzodiazepine ligand

Released:
DOI: 10.2210/pdb6s25/pdb
PDB entry 6s25 coloured by chain, front view.
PDB entry 6s25 coloured by chain, side view.
PDB entry 6s25 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Picaud S, Traquete R, Bernardes GJL, Tobias K, von Delft F, Arrowsmith CH, Edwards AM, Bountra C, Filippakopoulos P, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bromodomain-containing protein 4 Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 15.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60885 (Residues: 44-168; Coverage: 9%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain

Ligands and Environments

2 bound ligands:
Ligand EDO 3 x EDO
Ligand KSZ 1 x KSZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 37.247Å b: 44.228Å c: 78.512Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.125 0.124 0.145
Expression system: Escherichia coli BL21(DE3)

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