› 6sek

X-ray diffraction
2.7Å resolution

Crystal Structure of Ancestral Flavin-containing monooxygenase (FMO) 5

Released:
DOI: 10.2210/pdb6sek/pdb
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PDB entry 6sek coloured by chain, front view.
PDB entry 6sek coloured by chain, side view.
PDB entry 6sek coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Ancestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs.
Nicoll CR, Bailleul G, Fiorentini F, Mascotti ML, Fraaije MW, Mattevi A
Nat Struct Mol Biol 27 14-24 (2020)
PMID: 31873300

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167458 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ancestral Flavin-containing monooxygenase 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 533 amino acids
Theoretical weight: 60.36 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

5 bound ligands:
Ligand FAD 2 x FAD
Ligand NAP 2 x NAP
Ligand LMT 1 x LMT
Ligand EPE 2 x EPE
Ligand GLC 2 x GLC
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Unit cell:
a: 96.782Å b: 100.069Å c: 143.152Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.222 0.295
Expression system: Escherichia coli

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Citations

2 review citations

Ancestral sequence reconstruction as a tool to study the evolution of wood decaying fungi.
Ayuso-Fernández et al. (2022)
1 more

3 mentions without citation

Evolution of enzyme functionality in the flavin-containing monooxygenases.
Bailleul et al. (2023)
2 more

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