› 6shm

X-ray diffraction
1.9Å resolution

An inactive (D136A and D137A) variant of alpha-1,6-mannanase, GH76A of Salegentibacter sp. HEL1_6 in complex with alpha-1,6-mannotetrose

Released:
DOI: 10.2210/pdb6shm/pdb
PDB entry 6shm coloured by chain, front view.
PDB entry 6shm coloured by chain, side view.
PDB entry 6shm coloured by chain, top view.
Source organism: Salegentibacter sp. Hel_I_6
Primary publication:
Glycoside hydrolase from the GH76 family indicates that marine Salegentibacter sp. Hel_I_6 consumes alpha-mannan from fungi.
Solanki V, Krüger K, Crawford CJ, Pardo-Vargas A, Danglad-Flores J, Hoang KLM, Klassen L, Abbott DW, Seeberger PH, Amann RI, Teeling H, Hehemann JH
OpenAccess logo ISME J 16 1818-1830 (2022)
PMID: 35414716

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-288745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Mutant alpha-1,6-mannanase GH76A Chain: A
Molecule details ›
Chain: A
Length: 391 amino acids
Theoretical weight: 43.79 KDa
Source organism: Salegentibacter sp. Hel_I_6
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: A0A7M4DUB6 (Residues: 1-371; Coverage: 100%)
Gene name: GH76A
Sequence domains: Glycosyl hydrolase family 76

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
2 bound ligands:
Ligand MES 1 x MES
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Unit cell:
a: 37.15Å b: 85.31Å c: 95.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.121 0.118 0.172
Expression system: Escherichia coli K-12

Quick links

Citations

3 citation in other articles

Trait biases in microbial reference genomes.
Albright et al. (2023)
2 more

1 mention without citation

Glycoside hydrolase from the GH76 family indicates that marine Salegentibacter sp. Hel_I_6 consumes alpha-mannan from fungi.
Solanki et al. (2022)