› 6stj

X-ray diffraction
2.2Å resolution

Selective Affimers Recognize BCL-2 Family Proteins Through Non-Canonical Structural Motifs

Released:
DOI: 10.2210/pdb6stj/pdb
PDB entry 6stj coloured by chain, front view.
PDB entry 6stj coloured by chain, side view.
PDB entry 6stj coloured by chain, top view.
Source organisms:
Primary publication:
Selective Affimers Recognise the BCL-2 Family Proteins BCL-xL and MCL-1 through Noncanonical Structural Motifs*.
Miles JA, Hobor F, Trinh CH, Taylor J, Tiede C, Rowell PR, Jackson BR, Nadat FA, Ramsahye P, Kyle HF, Wicky BIM, Clarke J, Tomlinson DC, Wilson AJ, Edwards TA
OpenAccess logo Chembiochem 22 232-240 (2021)
PMID: 32961017

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-553456 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Induced myeloid leukemia cell differentiation protein Mcl-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 156 amino acids
Theoretical weight: 17.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07820 (Residues: 173-327; Coverage: 44%)
Gene names: BCL2L3, MCL1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Cystatin domain-containing protein Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 91 amino acids
Theoretical weight: 10.97 KDa
Source organism: saltans group
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2221
Unit cell:
a: 92.142Å b: 107.498Å c: 226.154Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.257 0.29
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Mechanism of activation and the rewired network: New drug design concepts.
Nussinov et al. (2022)
5 other articles