› 6sup

X-ray diffraction
2Å resolution

Crystal Structure of TcdB2-TccC3-Cdc42

Released:
DOI: 10.2210/pdb6sup/pdb
PDB entry 6sup coloured by chain, front view.
PDB entry 6sup coloured by chain, side view.
PDB entry 6sup coloured by chain, top view.
Source organisms:
Primary publication:
Towards the application of Tc toxins as a universal protein translocation system.
Roderer D, Schubert E, Sitsel O, Raunser S
OpenAccess logo Nat Commun 10 5263 (2019)
PMID: 31748551

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division control protein 42 homolog; TccC3; TcdB2 Chain: A
Molecule details ›
Chain: A
Length: 2128 amino acids
Theoretical weight: 240.83 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GF99 (Residues: 32-1471; Coverage: 98%)
  • Canonical: Q8GF97 (Residues: 35-678; Coverage: 67%)
  • Canonical: P60953 (Residues: 167-179; Coverage: 7%)
Gene names: CDC42, TccC3, tcdB2
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 96.35Å b: 156.55Å c: 179.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.287 0.287 0.32
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Challenges and approaches to studying pore-forming proteins.
Benton et al. (2021)
3 more

1 mention without citation

Diversity and function of arthropod endosymbiont toxins.
Massey et al. (2022)