› 6sw6

X-ray diffraction
2.85Å resolution

Crystal structure R264H mutant of the human S-adenosylmethionine synthetase 1

Released:
DOI: 10.2210/pdb6sw6/pdb
PDB entry 6sw6 coloured by chain, front view.
PDB entry 6sw6 coloured by chain, side view.
PDB entry 6sw6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of the dominant inheritance of hypermethioninemia associated with the Arg264His mutation in the MAT1A gene.
Panmanee J, Antonyuk SV, Hasnain SS
OpenAccess logo Acta Crystallogr D Struct Biol 76 594-607 (2020)
PMID: 32496220

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169403 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase isoform type-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 395 amino acids
Theoretical weight: 43.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00266 (Residues: 1-395; Coverage: 100%)
Gene names: AMS1, MAT1A, MATA1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 108.558Å b: 83.619Å c: 87.562Å
α: 90° β: 107.99° γ: 90°
R-values:
R R work R free
0.262 0.261 0.288
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Implications of divergence of methionine adenosyltransferase in archaea.
Chouhan et al. (2022)
2 more

1 mention without citation

Structural basis of the dominant inheritance of hypermethioninemia associated with the Arg264His mutation in the MAT1A gene.
Panmanee et al. (2020)