› 6tlj

Electron Microscopy
3.8Å resolution

Cryo-EM structure of the Anaphase-promoting complex/Cyclosome, in complex with the Mitotic checkpoint complex (APC/C-MCC) at 3.8 angstrom resolution

Released:
DOI: 10.2210/pdb6tlj/pdb
PDB entry 6tlj coloured by chain, front view.
PDB entry 6tlj coloured by chain, side view.
PDB entry 6tlj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A unique binding mode of Nek2A to the APC/C allows its ubiquitination during prometaphase.
Alfieri C, Tischer T, Barford D
OpenAccess logo EMBO Rep 21 e49831 (2020)
PMID: 32307883
Related structures: EMD-10516

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 23-mer (preferred)
PDBe Complex ID:
PDB-CPX-130028 (preferred)
Entry contents:
18 distinct polypeptide molecules
Macromolecules (18 distinct):
Anaphase-promoting complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 1944 amino acids
Theoretical weight: 216.78 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9H1A4 (Residues: 1-1944; Coverage: 100%)
Gene names: ANAPC1, TSG24
Sequence domains:
Anaphase-promoting complex subunit 11 Chain: B
Molecule details ›
Chain: B
Length: 84 amino acids
Theoretical weight: 9.85 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NYG5 (Residues: 1-84; Coverage: 100%)
Gene names: ANAPC11, HSPC214
Sequence domains: Anaphase-promoting complex subunit 11 RING-H2 finger
Cell division cycle protein 23 homolog Chains: C, P
Anaphase-promoting complex subunit 15 Chain: D
Anaphase-promoting complex subunit 16 Chain: E
Cell division cycle protein 27 homolog Chains: F, H
Anaphase-promoting complex subunit CDC26 Chains: G, W
Anaphase-promoting complex subunit 4 Chain: I
Cell division cycle protein 16 homolog Chains: J, K
Anaphase-promoting complex subunit 10 Chain: L
Anaphase-promoting complex subunit 13 Chain: M
Anaphase-promoting complex subunit 2 Chain: N
Anaphase-promoting complex subunit 5 Chain: O
Cell division cycle protein 20 homolog Chain: Q
Cell division cycle protein 20 homolog Chain: R
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta Chain: S
Anaphase-promoting complex subunit 7 Chains: X, Y
Mitotic spindle assembly checkpoint protein MAD2A Chain: Z

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-10516
Expression system: Trichoplusia ni

Quick links

Citations

3 review citations

On Broken Ne(c)ks and Broken DNA: The Role of Human NEKs in the DNA Damage Response.
Pavan et al. (2021)
2 more

6 mentions without citation

Using Budding Yeast to Identify Molecules That Block Cancer Cell 'Mitotic Slippage' Only in the Presence of Mitotic Poisons.
Schuyler et al. (2021)
5 more