› 6tmg

Electron Microscopy
2.8Å resolution

Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase dimer, membrane region model

Released:
DOI: 10.2210/pdb6tmg/pdb
PDB entry 6tmg coloured by chain, front view.
PDB entry 6tmg coloured by chain, side view.
PDB entry 6tmg coloured by chain, top view.
Source organism: Toxoplasma gondii GT1
Primary publication:
ATP synthase hexamer assemblies shape cristae of Toxoplasma mitochondria.
Mühleip A, Kock Flygaard R, Ovciarikova J, Lacombe A, Fernandes P, Sheiner L, Amunts A
OpenAccess logo Nat Commun 12 120 (2021)
PMID: 33402698
Related structures: EMD-10520

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 48-mer (preferred)
PDBe Complex ID:
PDB-CPX-102879 (preferred)
Entry contents:
24 distinct polypeptide molecules
Macromolecules (24 distinct):
ATPTG11 Chains: Q, q
Molecule details ›
Chains: Q, q
Length: 134 amino acids
Theoretical weight: 15.43 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: A0A125YPS4 (Residues: 1-134; Coverage: 100%)
Gene name: TGGT1_263990
ATPTG7 Chains: I, i
Molecule details ›
Chains: I, i
Length: 236 amino acids
Theoretical weight: 26.87 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: S7VXW3 (Residues: 1-236; Coverage: 100%)
Gene name: TGGT1_290710
ATPTG14 Chains: T, t
Molecule details ›
Chains: T, t
Length: 133 amino acids
Theoretical weight: 14.42 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: A0A125YLH9 (Residues: 1-133; Coverage: 100%)
Gene name: TGGT1_263080
ATPTG5 Chains: G, g
Molecule details ›
Chains: G, g
Length: 252 amino acids
Theoretical weight: 28.16 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: S7WD71 (Residues: 1-252; Coverage: 100%)
Gene name: TGGT1_270360
subunit k Chains: O, o
Molecule details ›
Chains: O, o
Length: 157 amino acids
Theoretical weight: 17.96 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: A0A125YTD7 (Residues: 1-157; Coverage: 100%)
Gene name: TGGT1_260180
Transmembrane protein Chains: K, k
Molecule details ›
Chains: K, k
Length: 224 amino acids
Theoretical weight: 24.56 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: A0A125YSI9 (Residues: 1-224; Coverage: 100%)
Gene name: TGGT1_310360
Transmembrane protein Chains: J, j
Molecule details ›
Chains: J, j
Length: 229 amino acids
Theoretical weight: 26.6 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: S7UQ82 (Residues: 1-229; Coverage: 100%)
Gene name: TGGT1_290030
Transmembrane protein Chains: S, s
Molecule details ›
Chains: S, s
Length: 128 amino acids
Theoretical weight: 14.92 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: A0A125YLN4 (Residues: 1-128; Coverage: 100%)
Gene name: TGGT1_225730
Transmembrane protein Chains: U, u
Molecule details ›
Chains: U, u
Length: 126 amino acids
Theoretical weight: 13.87 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: A0A125YRP0 (Residues: 1-126; Coverage: 100%)
Gene name: TGGT1_247410
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 Chains: H, h
Molecule details ›
Chains: H, h
Length: 239 amino acids
Theoretical weight: 27.48 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: A0A125YL08 (Residues: 1-239; Coverage: 100%)
Gene name: TGGT1_223040
Transmembrane protein Chains: E, e
Molecule details ›
Chains: E, e
Length: 325 amino acids
Theoretical weight: 35.16 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: A0A125YLR0 (Residues: 1-326; Coverage: 100%)
Gene name: TGGT1_218940
Transmembrane protein Chains: X, x
Molecule details ›
Chains: X, x
Length: 83 amino acids
Theoretical weight: 9.49 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: S7W180 (Residues: 1-76; Coverage: 76%)
Gene name: TGGT1_310180
subunit b Chains: B, b
Molecule details ›
Chains: B, b
Length: 571 amino acids
Theoretical weight: 64.81 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: S7V2T0 (Residues: 1-571; Coverage: 100%)
Gene name: TGGT1_231410
ATPTG12 Chains: R, r
Molecule details ›
Chains: R, r
Length: 134 amino acids
Theoretical weight: 15.4 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: A0A125YKF7 (Residues: 1-134; Coverage: 100%)
Gene name: TGGT1_245450
ATPTG10-like domain-containing protein Chains: P, p
Molecule details ›
Chains: P, p
Length: 138 amino acids
Theoretical weight: 15.17 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: A0A125YMA7 (Residues: 1-138; Coverage: 100%)
Gene name: TGGT1_214930
Sequence domains: ATPTG10-like
Transmembrane protein Chains: V, v
Molecule details ›
Chains: V, v
Length: 111 amino acids
Theoretical weight: 13.03 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: S7UQT7 (Residues: 1-111; Coverage: 100%)
Gene name: TGGT1_215610
Putative myosin heavy chain Chains: L, l
Molecule details ›
Chains: L, l
Length: 208 amino acids
Theoretical weight: 23.37 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: S7W7F1 (Residues: 1-208; Coverage: 100%)
Gene name: TGGT1_258060
Cytochrome c domain-containing protein Chains: C, c
Molecule details ›
Chains: C, c
Length: 398 amino acids
Theoretical weight: 46.03 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: S7W9J5 (Residues: 1-398; Coverage: 100%)
Gene name: TGGT1_246540
Sequence domains: Cytochrome C1 family
ATPTG2 Chains: D, d
Molecule details ›
Chains: D, d
Length: 310 amino acids
Theoretical weight: 35.24 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: A0A125YV76 (Residues: 1-310; Coverage: 100%)
Gene name: TGGT1_282180
Transmembrane protein Chains: M, m
Molecule details ›
Chains: M, m
Length: 205 amino acids
Theoretical weight: 22.34 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: A0A125YPQ4 (Residues: 1-205; Coverage: 100%)
Gene name: TGGT1_208440
CHCH domain-containing protein Chains: N, n
Molecule details ›
Chains: N, n
Length: 166 amino acids
Theoretical weight: 18.43 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: A0A125YUZ2 (Residues: 1-166; Coverage: 100%)
Gene name: TGGT1_285510
Thioredoxin domain-containing protein Chains: F, f
Molecule details ›
Chains: F, f
Length: 267 amino acids
Theoretical weight: 28.56 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: S7UVZ7 (Residues: 1-267; Coverage: 100%)
Gene name: TGGT1_201800
Sequence domains: Thioredoxin
Transmembrane protein Chains: W, w
Molecule details ›
Chains: W, w
Length: 106 amino acids
Theoretical weight: 11.59 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: S7VTI0 (Residues: 1-106; Coverage: 100%)
Gene name: TGGT1_211060
subunit d Chains: A, a
Molecule details ›
Chains: A, a
Length: 536 amino acids
Theoretical weight: 61.36 KDa
Source organism: Toxoplasma gondii GT1
UniProt:
  • Canonical: S7V493 (Residues: 134-669; Coverage: 80%)
Gene name: TGGT1_268835

Ligands and Environments

4 bound ligands:
Ligand PC1 6 x PC1
Ligand LMT 14 x LMT
Ligand CDL 18 x CDL
Ligand PEE 6 x PEE
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-10520

Quick links

Citations

10 review citations

Electron microscopy holdings of the Protein Data Bank: the impact of the resolution revolution, new validation tools, and implications for the future.
Burley et al. (2022)
9 more