› 6ue0

X-ray diffraction
1.89Å resolution

Crystal structure of dihydrodipicolinate synthase from Klebsiella pneumoniae bound to pyruvate

Released:
DOI: 10.2210/pdb6ue0/pdb
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PDB entry 6ue0 coloured by chain, front view.
PDB entry 6ue0 coloured by chain, side view.
PDB entry 6ue0 coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Mis-annotations of a promising antibiotic target in high-priority gram-negative pathogens.
Impey RE, Lee M, Hawkins DA, Sutton JM, Panjikar S, Perugini MA, Soares da Costa TP
FEBS Lett 594 1453-1463 (2020)
PMID: 31943170

Function and Biology Details

Reaction catalysed: 
Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-tetrahydrodipicolinate synthase Chains: AAA, BBB
Molecule details ›
Chains: AAA, BBB
Length: 326 amino acids
Theoretical weight: 34.82 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: W9BBZ5 (Residues: 1-292; Coverage: 100%)
Gene names: B5L96_12830, B6I68_04175, BANRA_01396, BL124_00003115, CP554_06510, DM078_04925, DW286_03605, E1814_08465, EA160_05765, EAO28_22650, EXT45_21445, FME62_14995, FXN67_06815, G4V31_13675, GJJ01_16410, GJJ08_007305, GJJ18_14280, GNF00_21800, H3G96_020425, IE978_04150, IE979_07200, IE990_15570, JMZ77_06720, KPZU09_73890, LS45_12065, NCTC11679_01461, NCTC13443_05599, NCTC204_03901, NCTC5051_03824, NCTC5052_04771, NCTC5053_05998, NCTC8849_04503, NCTC9128_06594, NCTC9601_01528, NCTC9617_05876, NCTC9637_02121, NCTC9645_04189, Q6294_16650, SAMEA104567804_00446, SAMEA2273558_01374, SAMEA3499874_02644, SAMEA3499901_02240, SAMEA3515122_01983, SAMEA3538828_02368, SAMEA3649591_01008, SAMEA3720909_00317, SAMEA3729652_03147, SAMEA4364603_01487, SAMEA4873632_02362, dapA, dapA_1, dapA_2, dapA_3
Sequence domains: Dihydrodipicolinate synthetase family

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand CL 6 x CL
1 modified residue:
Ligand KPI 2 x KPI

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21212
Unit cell:
a: 105.634Å b: 114.23Å c: 55.298Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.198
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Overcoming Intrinsic and Acquired Resistance Mechanisms Associated with the Cell Wall of Gram-Negative Bacteria.
Impey et al. (2020)
1 more

1 mention without citation

Mis-annotations of a promising antibiotic target in high-priority gram-negative pathogens.
Impey et al. (2020)

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