6vtt

Electron Microscopy
3.7Å resolution

Cryo-EM Structure of CAP256-VRC26.25 Fab bound to HIV-1 Env trimer CAP256.wk34.c80 SOSIP.RnS2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-207324 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (10 distinct):
Envelope glycoprotein gp160 Chains: E, F, G
Molecule details ›
Chains: E, F, G
Length: 471 amino acids
Theoretical weight: 53.32 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A0N9FF17 (Residues: 29-497; Coverage: 55%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
VRC26.25 Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 257 amino acids
Theoretical weight: 28.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 23.05 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: A2NUT2 (Residues: 20-116, 117-235; Coverage: 100%)
Sequence domains:
Envelope glycoprotein gp41 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 154 amino acids
Theoretical weight: 17.36 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-21383
Expression system: Homo sapiens