› 6vzj

Electron Microscopy
4.1Å resolution

Escherichia coli transcription-translation complex A1 (TTC-A1) containing mRNA with a 15 nt long spacer, fMet-tRNAs at E-site and P-site, and lacking transcription factor NusG

Released:
DOI: 10.2210/pdb6vzj/pdb
PDB entry 6vzj coloured by chain, front view.
PDB entry 6vzj coloured by chain, side view.
PDB entry 6vzj coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of transcription-translation coupling.
Wang C, Molodtsov V, Firlar E, Kaelber JT, Blaha G, Su M, Ebright RH
Science 369 1359-1365 (2020)
PMID: 32820061
Related structures: EMD-21494

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 65-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238556 (preferred)
Entry contents:
56 distinct polypeptide molecules
2 distinct DNA molecules
5 distinct RNA molecules
Macromolecules (63 distinct):
Large ribosomal subunit protein bL21 Chain: 0
Molecule details ›
Chain: 0
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL22 Chain: 1
Molecule details ›
Chain: 1
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Large ribosomal subunit protein uL23 Chain: 2
Large ribosomal subunit protein uL24 Chain: 3
Large ribosomal subunit protein bL25 Chain: 4
Large ribosomal subunit protein uL10 Chain: 9
DNA-directed RNA polymerase subunit beta Chain: AA
DNA-directed RNA polymerase subunit alpha Chains: AC, AD
DNA-directed RNA polymerase subunit beta' Chain: AE
Small ribosomal subunit protein bS18 Chain: C
Small ribosomal subunit protein bS20 Chain: E
Small ribosomal subunit protein bS21 Chain: F
Small ribosomal subunit protein uS2 Chain: G
Small ribosomal subunit protein bS1 Chain: H
Small ribosomal subunit protein uS3 Chain: I
Small ribosomal subunit protein uS4 Chain: J
Small ribosomal subunit protein uS5 Chain: K
Small ribosomal subunit protein bS6 Chain: L
Small ribosomal subunit protein uS7 Chain: M
Small ribosomal subunit protein uS8 Chain: N
Small ribosomal subunit protein uS9 Chain: O
Small ribosomal subunit protein uS10 Chain: P
Small ribosomal subunit protein uS11 Chain: Q
Small ribosomal subunit protein uS12 Chain: R
Small ribosomal subunit protein uS14 Chain: S
Small ribosomal subunit protein uS15 Chain: T
Small ribosomal subunit protein bS16 Chain: U
Small ribosomal subunit protein uS17 Chain: V
Small ribosomal subunit protein uS19 Chain: W
Small ribosomal subunit protein uS13 Chain: X
Large ribosomal subunit protein uL11 Chain: Y
Large ribosomal subunit protein bL12 Chain: Z
Large ribosomal subunit protein bL27 Chain: b
Large ribosomal subunit protein bL28 Chain: c
Large ribosomal subunit protein uL29 Chain: e
Large ribosomal subunit protein uL30 Chain: f
Large ribosomal subunit protein bL31 Chain: g
Large ribosomal subunit protein uL2 Chain: h
Large ribosomal subunit protein bL32 Chain: i
Large ribosomal subunit protein uL3 Chain: j
Large ribosomal subunit protein bL33 Chain: k
Large ribosomal subunit protein uL4 Chain: l
Large ribosomal subunit protein bL34 Chain: m
Large ribosomal subunit protein uL5 Chain: n
Large ribosomal subunit protein bL35 Chain: o
Large ribosomal subunit protein uL6 Chain: p
Large ribosomal subunit protein bL36A Chain: q
Large ribosomal subunit protein bL9 Chain: r
Large ribosomal subunit protein uL13 Chain: s
Large ribosomal subunit protein uL14 Chain: t
Large ribosomal subunit protein uL15 Chain: u
Large ribosomal subunit protein uL16 Chain: v
Large ribosomal subunit protein bL17 Chain: w
Large ribosomal subunit protein uL18 Chain: x
Large ribosomal subunit protein bL19 Chain: y
Large ribosomal subunit protein bL20 Chain: z
NT DNA Chain: 5
T DNA Chain: 6
mRNA with 27 nt long spacer Chain: 7
E-site and P-site tRNA (fMet) Chains: A, B
16S ribosomal RNA Chain: D
23S ribosomal RNA Chain: a
5S ribosomal RNA Chain: d

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-21494
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

20 review citations

Translational Control by Ribosome Pausing in Bacteria: How a Non-uniform Pace of Translation Affects Protein Production and Folding.
Samatova et al. (2020)
19 more

1 mention without citation

Structural basis of transcription-translation coupling.
Wang et al. (2020)