› 6weo

X-ray diffraction
2.6Å resolution

IL-22 Signaling Complex with IL-22R1 and IL-10Rbeta

Released:
DOI: 10.2210/pdb6weo/pdb
PDB entry 6weo coloured by chain, front view.
PDB entry 6weo coloured by chain, side view.
PDB entry 6weo coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The tissue protective functions of interleukin-22 can be decoupled from pro-inflammatory actions through structure-based design.
Saxton RA, Henneberg LT, Calafiore M, Su L, Jude KM, Hanash AM, Garcia KC
Immunity 54 660-672.e9 (2021)
PMID: 33852830

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-178867 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (11 distinct):
Interleukin-10 receptor subunit beta Chains: 0, 3, 6, 9, C, E, H, K, O, R, U, X
Molecule details ›
Chains: 0, 3, 6, 9, C, E, H, K, O, R, U, X
Length: 204 amino acids
Theoretical weight: 23.81 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q61190 (Residues: 20-220; Coverage: 61%)
Gene names: Crfb4, Il10rb
Sequence domains:
Interleukin-22 receptor subunit alpha-1 Chains: 1, 4, 7, A, B, F, I, M, P, S, V, Y
Molecule details ›
Chains: 1, 4, 7, A, B, F, I, M, P, S, V, Y
Length: 204 amino acids
Theoretical weight: 23.26 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q80XZ4 (Residues: 24-224; Coverage: 36%)
Gene name: Il22ra1
Sequence domains: Tissue factor
Interleukin-22 Chains: 2, 5, 8, D, G, J, L, N, Q, T, W, Z
Molecule details ›
Chains: 2, 5, 8, D, G, J, L, N, Q, T, W, Z
Length: 149 amino acids
Theoretical weight: 16.99 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9JJY9 (Residues: 34-179; Coverage: 100%)
Gene names: Il22, Il22a, Iltif, Iltifa
Sequence domains: Interleukin 22 IL-10-related T-cell-derived-inducible factor

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand GOL 7 x GOL
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P1
Unit cell:
a: 134.487Å b: 145.219Å c: 152.038Å
α: 71.062° β: 81.836° γ: 62.475°
R-values:
R R work R free
0.237 0.236 0.296
Expression system: Trichoplusia ni

Quick links

Citations

9 review citations

T Cell Subsets in Graft Versus Host Disease and Graft Versus Tumor.
Jiang et al. (2021)
8 more

3 mentions without citation

Emerging principles of cytokine pharmacology and therapeutics.
Saxton et al. (2023)
2 more