› 6wwa

X-ray diffraction
3.8Å resolution

Crystal structure of human SHLD2-SHLD3-REV7 complex

Released:
DOI: 10.2210/pdb6wwa/pdb
PDB entry 6wwa coloured by chain, front view.
PDB entry 6wwa coloured by chain, side view.
PDB entry 6wwa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular mechanisms of assembly and TRIP13-mediated remodeling of the human Shieldin complex.
Xie W, Wang S, Wang J, de la Cruz MJ, Xu G, Scaltriti M, Patel DJ
OpenAccess logo Proc Natl Acad Sci U S A 118 (2021)
PMID: 33597306

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180591 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitotic spindle assembly checkpoint protein MAD2B Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 211 amino acids
Theoretical weight: 24.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UI95 (Residues: 2-211; Coverage: 100%)
Gene names: MAD2B, MAD2L2, REV7
Sequence domains: HORMA domain
Shieldin complex subunit 2; Shieldin complex subunit 3 Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 99 amino acids
Theoretical weight: 10.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86V20 (Residues: 5-19; Coverage: 2%)
  • Canonical: Q6ZNX1 (Residues: 2-58; Coverage: 23%)
Gene names: FAM35A, FLJ26957, RINN1, RINN2, SHLD2, SHLD3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I432
Unit cell:
a: 331.842Å b: 331.842Å c: 331.842Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.239 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

REV7 directs DNA repair pathway choice.
Clairmont et al. (2021)
4 more

2 mentions without citation

Molecular mechanisms of assembly and TRIP13-mediated remodeling of the human Shieldin complex.
Xie et al. (2021)
1 more