› 6x9l

X-ray diffraction
2.52Å resolution

Crystal Structure of Aldehyde Dehydrogenase C (AldC) mutant (C291A) from Pseudomonas syringae in complexed with NAD+ and Octanal

Released:
DOI: 10.2210/pdb6x9l/pdb
Model geometry
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PDB entry 6x9l coloured by chain, front view.
PDB entry 6x9l coloured by chain, side view.
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Source organism: Pseudomonas syringae pv. tomato str. DC3000
Primary publication:
The plant pathogen enzyme AldC is a long-chain aliphatic aldehyde dehydrogenase.
Lee SG, Harline K, Abar O, Akadri SO, Bastian AG, Chen HS, Duan M, Focht CM, Groziak AR, Kao J, Kottapalli JS, Leong MC, Lin JJ, Liu R, Luo JE, Meyer CM, Mo AF, Pahng SH, Penna V, Raciti CD, Srinath A, Sudhakar S, Tang JD, Cox BR, Holland CK, Cascella B, Cruz W, McClerkin SA, Kunkel BN, Jez JM
J Biol Chem 295 13914-13926 (2020)
PMID: 32796031

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183398 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 491 amino acids
Theoretical weight: 51.78 KDa
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Expression system: Escherichia coli
UniProt:
  • Canonical: Q87YZ5 (Residues: 1-491; Coverage: 100%)
Gene name: PSPTO_3644
Sequence domains: Aldehyde dehydrogenase family

Ligands and Environments

2 bound ligands:
Ligand OYA 2 x OYA
Ligand NAD 2 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 92.193Å b: 92.17Å c: 231.357Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.197
Expression system: Escherichia coli

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Citations

1 review citation

Recent Development on Plant Aldehyde Dehydrogenase Enzymes and Their Functions in Plant Development and Stress Signaling.
Tola et al. (2020)
2 other articles

1 mention without citation

The plant pathogen enzyme AldC is a long-chain aliphatic aldehyde dehydrogenase.
Lee et al. (2020)

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