› 6xds

X-ray diffraction
1.47Å resolution

Crystal structure of MBP-TREM2 Ig domain fusion with fragment, 2-((4-bromophenyl)amino)ethan-1-ol

Released:
DOI: 10.2210/pdb6xds/pdb
PDB entry 6xds coloured by chain, front view.
PDB entry 6xds coloured by chain, side view.
PDB entry 6xds coloured by chain, top view.
Source organisms:
Primary publication:
Development of a robust crystallization platform for immune receptor TREM2 using a crystallization chaperone strategy.
Byrne NJ, Lee AC, Kostas J, Reid JC, Partridge AT, So SS, Cowan JE, Abeywickrema P, Huang H, Zebisch M, Barker JJ, Soisson SM, Brooun A, Su HP
Protein Expr Purif 179 105796 (2021)
PMID: 33221505

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142599 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Triggering receptor expressed on myeloid cells 2 Chain: A
Molecule details ›
Chain: A
Length: 492 amino acids
Theoretical weight: 54.17 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: Q9NZC2 (Residues: 17-135; Coverage: 55%)
Gene names: JW3994, TREM2, b4034, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand V5S 1 x V5S
Ligand DMS 3 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 73.84Å b: 80.35Å c: 103.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.208
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

Targeting TREM2 for Parkinson's Disease: Where to Go?
Li et al. (2021)

3 mentions without citation

Structural biology of cell surface receptors implicated in Alzheimer's disease.
Hermans et al. (2022)
2 more