› 6xn2

X-ray diffraction
1.65Å resolution

Crystal structure of the GH43_1 enzyme from Xanthomonas citri complexed with xylotriose

Released:
DOI: 10.2210/pdb6xn2/pdb
PDB entry 6xn2 coloured by chain, front view.
PDB entry 6xn2 coloured by chain, side view.
PDB entry 6xn2 coloured by chain, top view.
Source organism: Xanthomonas citri pv. citri str. 306
Primary publication:
Two distinct catalytic pathways for GH43 xylanolytic enzymes unveiled by X-ray and QM/MM simulations.
Morais MAB, Coines J, Domingues MN, Pirolla RAS, Tonoli CCC, Santos CR, Correa JBL, Gozzo FC, Rovira C, Murakami MT
OpenAccess logo Nat Commun 12 367 (2021)
PMID: 33446650

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241524 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Xylosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 364 amino acids
Theoretical weight: 40.23 KDa
Source organism: Xanthomonas citri pv. citri str. 306
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
4 bound ligands:
Ligand CA 2 x CA
Ligand XYP 1 x XYP
Ligand PEG 9 x PEG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: C2221
Unit cell:
a: 63.593Å b: 165.874Å c: 158.756Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.201
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Asparagine Tautomerization in Glycosyltransferase Catalysis. The Molecular Mechanism of Protein O-Fucosyltransferase 1.
Piniello et al. (2021)
4 more

1 mention without citation

Molecular mechanism of active Cas7-11 in processing CRISPR RNA and interfering target RNA.
Goswami et al. (2022)