› 6xog

X-ray diffraction
1.98Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159021 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
SUMO-activating enzyme subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 346 amino acids
Theoretical weight: 38.5 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UBE0 (Residues: 1-346; Coverage: 100%)
Gene names: AOS1, SAE1, SUA1, UBLE1A
Sequence domains: ThiF family
SUMO-activating enzyme subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 640 amino acids
Theoretical weight: 71.31 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UBT2 (Residues: 1-640; Coverage: 100%)
Gene names: HRIHFB2115, SAE2, UBA2, UBLE1B
Sequence domains:
Small ubiquitin-related modifier 1 Chain: C
Molecule details ›
Chain: C
Length: 101 amino acids
Theoretical weight: 11.58 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63165 (Residues: 1-101; Coverage: 100%)
Gene names: OK/SW-cl.43, SMT3C, SMT3H3, SUMO1, UBL1
Sequence domains: Ubiquitin-2 like Rad60 SUMO-like

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand ZN 1 x ZN
Ligand VAY 1 x VAY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 57.9Å b: 72.422Å c: 126.87Å
α: 90° β: 93.858° γ: 90°
R-values:
R R work R free
0.208 0.206 0.24
Expression system: Spodoptera frugiperda

Quick links

Citations

21 review citations

Signalling mechanisms and cellular functions of SUMO.
Vertegaal ACO. (2022)
20 more