› 6yhr

X-ray diffraction
2.2Å resolution

Crystal structure of Werner syndrome helicase

Released:
DOI: 10.2210/pdb6yhr/pdb
PDB entry 6yhr coloured by chain, front view.
PDB entry 6yhr coloured by chain, side view.
PDB entry 6yhr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the helicase core of Werner helicase, a key target in microsatellite instability cancers.
Newman JA, Gavard AE, Lieb S, Ravichandran MC, Hauer K, Werni P, Geist L, Böttcher J, Engen JR, Rumpel K, Samwer M, Petronczki M, Gileadi O
OpenAccess logo Life Sci Alliance 4 (2021)
PMID: 33199508

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN Chain: A
Molecule details ›
Chain: A
Length: 585 amino acids
Theoretical weight: 66.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14191 (Residues: 517-1093; Coverage: 40%)
Gene names: RECQ3, RECQL2, WRN
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 54.595Å b: 90.627Å c: 138.233Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.236
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Probing the structure and function of polymerase θ helicase-like domain.
Vanson et al. (2022)
1 more

5 mentions without citation

Repair and tolerance of DNA damage at the replication fork: A structural perspective.
Eichman BF. (2023)
4 more