› 6yia

X-ray diffraction
1.3Å resolution

14-3-3 sigma in complex with SMAD2 pS465 peptide

Released:
DOI: 10.2210/pdb6yia/pdb
PDB entry 6yia coloured by chain, front view.
PDB entry 6yia coloured by chain, side view.
PDB entry 6yia coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kiehstaller S, Graf S, Hennig S

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-276197 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Mothers against decapentaplegic homolog 2 Chain: P
Molecule details ›
Chain: P
Length: 11 amino acids
Theoretical weight: 1.25 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15796 (Residues: 459-467; Coverage: 2%)
Gene names: MADH2, MADR2, SMAD2

Ligands and Environments

5 bound ligands:
Ligand MG 5 x MG
Ligand GOL 1 x GOL
Ligand CL 1 x CL
Ligand CA 1 x CA
Ligand NA 1 x NA
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 82.05Å b: 111.6Å c: 62.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.145 0.182
Expression systems:
  • Escherichia coli
  • Not provided

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