› 6yp1

X-ray diffraction
1.2Å resolution

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157519 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase 1 Chain: AAA
Molecule details ›
Chain: AAA
Length: 402 amino acids
Theoretical weight: 44.61 KDa
Source organism: Humicola insolens
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: P56680 (Residues: 1-402; Coverage: 100%)
Gene name: CEL7B
Sequence domains: Glycosyl hydrolase family 7

Ligands and Environments

4 bound ligands:
Ligand GOL 5 x GOL
Ligand SO4 9 x SO4
Ligand ACM 1 x ACM
Ligand NAG 2 x NAG
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P42212
Unit cell:
a: 92.31Å b: 92.31Å c: 100.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.118 0.117 0.135
Expression system: Aspergillus oryzae

Quick links

Citations

1 review citation

Activity-based probes in pathogenic bacteria: Investigating drug targets and molecule specificity.
Lembke et al. (2023)
9 other articles

1 mention without citation

Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases.
de Boer et al. (2020)