› 6z06

X-ray diffraction
3.5Å resolution

Crystal structure of Puumala virus Gc in complex with Fab 4G2

Released:
DOI: 10.2210/pdb6z06/pdb
PDB entry 6z06 coloured by chain, front view.
PDB entry 6z06 coloured by chain, side view.
PDB entry 6z06 coloured by chain, top view.
Source organism: Myodes glareolus
Primary publication:
Molecular rationale for antibody-mediated targeting of the hantavirus fusion glycoprotein.
Rissanen I, Stass R, Krumm SA, Seow J, Hulswit RJ, Paesen GC, Hepojoki J, Vapalahti O, Lundkvist Å, Reynard O, Volchkov V, Doores KJ, Huiskonen JT, Bowden TA
OpenAccess logo Elife 9 (2020)
PMID: 33349334

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212857 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab 4G2 Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 231 amino acids
Theoretical weight: 25.02 KDa
Source organism: Myodes glareolus
Expression system: Homo sapiens
Fab 4G2 Light chain Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.88 KDa
Source organism: Myodes glareolus
Expression system: Homo sapiens
Glycoprotein Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 49.83 KDa
UniProt:
  • Canonical: Q9WJ31 (Residues: 659-1105; Coverage: 39%)
Gene name: gpc
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 97.213Å b: 50.449Å c: 124.446Å
α: 90° β: 107.66° γ: 90°
R-values:
R R work R free
0.22 0.217 0.264
Expression system: Homo sapiens

Quick links

Citations

1 review citation

Recent Advances in Bunyavirus Glycoprotein Research: Precursor Processing, Receptor Binding and Structure.
Hulswit et al. (2021)
8 other articles

2 mentions without citation

The Input of Structural Vaccinology in the Search for Vaccines against Bunyaviruses.
Serris A. (2021)
1 more