› 6zl4

X-ray diffraction
3Å resolution

the structure of glutamate transporter homologue GltTk in complex with the photo switchable compound (cis)

Released:
DOI: 10.2210/pdb6zl4/pdb
PDB entry 6zl4 coloured by chain, front view.
PDB entry 6zl4 coloured by chain, side view.
PDB entry 6zl4 coloured by chain, top view.
Source organism: Thermococcus kodakarensis
Primary publication:
Structural Aspects of Photopharmacology: Insight into the Binding of Photoswitchable and Photocaged Inhibitors to the Glutamate Transporter Homologue.
Arkhipova V, Fu H, Hoorens MWH, Trinco G, Lameijer LN, Marin E, Feringa BL, Poelarends GJ, Szymanski W, Slotboom DJ, Guskov A
OpenAccess logo J Am Chem Soc 143 1513-1520 (2021)
PMID: 33449695

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proton/glutamate symporter, SDF family Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 438 amino acids
Theoretical weight: 46.69 KDa
Source organism: Thermococcus kodakarensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JID0 (Residues: 1-430; Coverage: 100%)
Gene name: TK0986
Sequence domains: Sodium:dicarboxylate symporter family

Ligands and Environments

7 bound ligands:
Ligand QM5 3 x QM5
Ligand DMU 3 x DMU
Ligand PG4 3 x PG4
Ligand PGE 6 x PGE
Ligand NA 9 x NA
Ligand PEG 14 x PEG
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P3221
Unit cell:
a: 117.21Å b: 117.21Å c: 309.41Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.256 0.254 0.288
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Photopharmacology of Ion Channels through the Light of the Computational Microscope.
Nin-Hill et al. (2021)
10 other articles

1 mention without citation

Structural Aspects of Photopharmacology: Insight into the Binding of Photoswitchable and Photocaged Inhibitors to the Glutamate Transporter Homologue.
Arkhipova et al. (2021)