› 6zpq

X-ray diffraction
1.85Å resolution

Crystal structure of the open conformation of Angiotensin-1 converting enzyme N-domain.

Released:
DOI: 10.2210/pdb6zpq/pdb
PDB entry 6zpq coloured by chain, front view.
PDB entry 6zpq coloured by chain, side view.
PDB entry 6zpq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Angiotensin-converting enzyme open for business: structural insights into the subdomain dynamics.
Cozier GE, Lubbe L, Sturrock ED, Acharya KR
OpenAccess logo FEBS J 288 2238-2256 (2021)
PMID: 33067882

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Angiotensin-converting enzyme, soluble form Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 629 amino acids
Theoretical weight: 72.61 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P12821 (Residues: 30-657; Coverage: 49%)
Gene names: ACE, DCP, DCP1
Sequence domains: Angiotensin-converting enzyme

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
12 bound ligands:
Ligand BO3 8 x BO3
Ligand BCN 5 x BCN
Ligand PEG 4 x PEG
Ligand EDO 14 x EDO
Ligand PGE 5 x PGE
Ligand ZN 4 x ZN
Ligand CL 4 x CL
Ligand MG 3 x MG
Ligand CA 4 x CA
Ligand NAG 1 x NAG
Ligand MXE 2 x MXE
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 74.683Å b: 99.934Å c: 128.701Å
α: 97.756° β: 90.206° γ: 111.057°
R-values:
R R work R free
0.185 0.184 0.214
Expression system: Cricetulus griseus

Quick links

Citations

3 review citations

ACE2 and Innate Immunity in the Regulation of SARS-CoV-2-Induced Acute Lung Injury: A Review.
Qu et al. (2021)
2 more

4 mentions without citation

ACE2 and ACE: structure-based insights into mechanism, regulation and receptor recognition by SARS-CoV.
Lubbe et al. (2020)
3 more