› 6a3a

X-ray diffraction
2.3Å resolution

MVM NES mutant Nm2 in complex with CRM1-Ran-RanBP1

Released:
DOI: 10.2210/pdb6a3a/pdb
Model geometry
Fit model/data
PDB entry 6a3a coloured by chain, front view.
PDB entry 6a3a coloured by chain, side view.
PDB entry 6a3a coloured by chain, top view.
Source organisms:
Primary publication:
Cancer Therapy with Nanoparticle-Medicated Intracellular Expression of Peptide CRM1-Inhibitor.
Sui M, Xiong M, Li Y, Zhou Q, Shen X, Jia D, Gou M, Sun Q
OpenAccess logo Int J Nanomedicine 16 2833-2847 (2021)
PMID: 33883894

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-143608 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
GTP-binding nuclear protein Ran Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 26.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62826 (Residues: 1-216; Coverage: 100%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
Ran-specific GTPase-activating protein 1 Chain: B
Molecule details ›
Chain: B
Length: 143 amino acids
Theoretical weight: 16.52 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41920 (Residues: 62-201; Coverage: 70%)
Gene names: CST20, HTN1, SFO1, YD8119.08, YDR002W, YRB1
Sequence domains: RanBP1 domain
Exportin-1 Chain: C
Non-structural protein NS2 Chain: D

Ligands and Environments

5 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand EDO 1 x EDO
Ligand GOL 1 x GOL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P43212
Unit cell:
a: 106.65Å b: 106.65Å c: 304.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Nanoparticles as Physically- and Biochemically-Tuned Drug Formulations for Cancers Therapy.
Foglizzo et al. (2022)