› 6a40

X-ray diffraction
1.8Å resolution

complex structure of Alginate lyase AlyF-OU02 with G4

Released:
DOI: 10.2210/pdb6a40/pdb
PDB entry 6a40 coloured by chain, front view.
PDB entry 6a40 coloured by chain, side view.
PDB entry 6a40 coloured by chain, top view.
Source organism: Vibrio splendidus
Primary publication:
Structural insights into a novel Ca2+-independent PL-6 alginate lyase from Vibrio OU02 identify the possible subsites responsible for product distribution.
Lyu Q, Zhang K, Shi Y, Li W, Diao X, Liu W
Biochim Biophys Acta Gen Subj 1863 1167-1176 (2019)
PMID: 31004719

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
alginate lyase AlyF-OU02 Chain: A
Molecule details ›
Chain: A
Length: 536 amino acids
Theoretical weight: 59.08 KDa
Source organism: Vibrio splendidus
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: LGU
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P61
Unit cell:
a: 106.212Å b: 106.212Å c: 100.846Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.166 0.199
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure Characteristics, Biochemical Properties, and Pharmaceutical Applications of Alginate Lyases.
Gao et al. (2021)
1 more

4 mentions without citation

Bacterial alginate metabolism: an important pathway for bioconversion of brown algae.
Zhang et al. (2021)
3 more