› 6a7v

X-ray diffraction
1.67Å resolution

Crystal structure of Mycobacterium tuberculosis VapBC11 toxin-antitoxin complex

Released:
DOI: 10.2210/pdb6a7v/pdb
Model geometry
Fit model/data
PDB entry 6a7v coloured by chain, front view.
PDB entry 6a7v coloured by chain, side view.
PDB entry 6a7v coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural, functional and biological insights into the role of Mycobacterium tuberculosis VapBC11 toxin-antitoxin system: targeting a tRNase to tackle mycobacterial adaptation.
Deep A, Tiwari P, Agarwal S, Kaundal S, Kidwai S, Singh R, Thakur KG
OpenAccess logo Nucleic Acids Res 46 11639-11655 (2018)
PMID: 30329074

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161404 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease VapC11 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 139 amino acids
Theoretical weight: 15.25 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WFA5 (Residues: 2-134; Coverage: 99%)
Gene names: MTCY48.04c, Rv1561, vapC11
Sequence domains: PIN domain
Antitoxin VapB11 Chains: D, H, K, U
Molecule details ›
Chains: D, H, K, U
Length: 62 amino acids
Theoretical weight: 6.9 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WLU3 (Residues: 7-68; Coverage: 86%)
Gene names: MTCY48.05c, Rv1560, vapB11
Sequence domains: Bacterial antitoxin of type II TA system, VapB

Ligands and Environments

2 bound ligands:
Ligand 1PE 1 x 1PE
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 50.555Å b: 127.007Å c: 151.207Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.209
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Biological Functions of Type II Toxin-Antitoxin Systems in Bacteria.
Kamruzzaman et al. (2021)
1 more

5 mentions without citation

Modulators of protein-protein interactions as antimicrobial agents.
Kahan et al. (2021)
4 more