6a8o

X-ray diffraction
2.77Å resolution

Crystal structures of the serine protease domain of murine plasma kallikrein with peptide inhibitor mupain-1-16

Released:

Function and Biology Details

Reaction catalysed:
Cleaves selectively Arg-|-Xaa and Lys-|-Xaa bonds, including Lys-|-Arg and Arg-|-Ser bonds in (human) kininogen to release bradykinin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-150684 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Plasma kallikrein light chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 27.65 KDa
Source organism: Mus musculus
Expression system: Pichia
UniProt:
  • Canonical: P26262 (Residues: 391-638; Coverage: 40%)
Gene names: Klk3, Klkb1, Pk
Sequence domains: Trypsin
peptide inhibitor, Chain: P
Molecule details ›
Chain: P
Length: 10 amino acids
Theoretical weight: 1.11 KDa
Source organism: Synthemiopsis
Expression system: Synthesiomyia

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 71.26Å b: 82.45Å c: 105.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.212 0.3
Expression systems:
  • Pichia
  • Synthesiomyia