› 6aje

X-ray diffraction
3.65Å resolution

Crystal structure of DHODH in complex with ferulenol from Eimeria tenella

Released:
DOI: 10.2210/pdb6aje/pdb
PDB entry 6aje coloured by chain, front view.
PDB entry 6aje coloured by chain, side view.
PDB entry 6aje coloured by chain, top view.
Source organism: Eimeria tenella
Primary publication:
Structural and Biochemical Features of Eimeria tenella Dihydroorotate Dehydrogenase, a Potential Drug Target.
Sato D, Hartuti ED, Inaoka DK, Sakura T, Amalia E, Nagahama M, Yoshioka Y, Tsuji N, Nozaki T, Kita K, Harada S, Matsubayashi M, Shiba T
OpenAccess logo Genes (Basel) 11 (2020)
PMID: 33297567

Function and Biology Details

Reaction catalysed: 
(S)-dihydroorotate + a quinone = orotate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotate dehydrogenase (quinone), mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 436 amino acids
Theoretical weight: 48.79 KDa
Source organism: Eimeria tenella
Expression system: Escherichia coli
UniProt:
  • Canonical: U6KL66 (Residues: 1-414; Coverage: 100%)
Gene name: ETH_00004975
Sequence domains: Dihydroorotate dehydrogenase

Ligands and Environments


Cofactor: Ligand FMN 4 x FMN
2 bound ligands:
Ligand ORO 4 x ORO
Ligand 9AU 4 x 9AU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P63
Unit cell:
a: 132.984Å b: 132.984Å c: 214.998Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.221 0.303
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Biochemical Studies of Mitochondrial Malate: Quinone Oxidoreductase from Toxoplasma gondii.
Acharjee et al. (2021)
3 more

1 mention without citation

Structural and Biochemical Features of Eimeria tenella Dihydroorotate Dehydrogenase, a Potential Drug Target.
Sato et al. (2020)