› 6aos

X-ray diffraction
2.3Å resolution

Crystal structure of the A/Brisbane/10/2007 (H3N2) influenza virus hemagglutinin L194P mutant in complex with 3'-SLNLN

Released:
DOI: 10.2210/pdb6aos/pdb
PDB entry 6aos coloured by chain, front view.
PDB entry 6aos coloured by chain, side view.
PDB entry 6aos coloured by chain, top view.
Source organism: Influenza A virus (A/Brisbane/10/2007(H3N2))
Primary publication:
A structural explanation for the low effectiveness of the seasonal influenza H3N2 vaccine.
Wu NC, Zost SJ, Thompson AJ, Oyen D, Nycholat CM, McBride R, Paulson JC, Hensley SE, Wilson IA
OpenAccess logo PLoS Pathog 13 e1006682 (2017)
PMID: 29059230

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111174 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.91 KDa
Source organism: Influenza A virus (A/Brisbane/10/2007(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: I6UCL3 (Residues: 11-329; Coverage: 97%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin (Ha1 Chain); Chain: A; domain 1
Hemagglutinin Chain: B
Molecule details ›
Chain: B
Length: 174 amino acids
Theoretical weight: 19.95 KDa
Source organism: Influenza A virus (A/Brisbane/10/2007(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: C3PR70 (Residues: 346-519; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, GAL, SIA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: R32
Unit cell:
a: 100.401Å b: 100.401Å c: 383.34Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.206 0.252
Expression system: Trichoplusia ni

Quick links

Citations

34 review citations

Advances in mRNA Vaccines for Infectious Diseases.
Zhang et al. (2019)
33 more

1 mention without citation

Preventing an Antigenically Disruptive Mutation in Egg-Based H3N2 Seasonal Influenza Vaccines by Mutational Incompatibility.
Wu et al. (2019)