› 6ape

X-ray diffraction
1.45Å resolution

Crystal Structure of Bifunctional protein FolD from Helicobacter pylori

Released:
DOI: 10.2210/pdb6ape/pdb
PDB entry 6ape coloured by chain, front view.
PDB entry 6ape coloured by chain, side view.
PDB entry 6ape coloured by chain, top view.
Source organism: Helicobacter pylori G27
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reactions catalysed: 
5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH
5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-110281 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein FolD Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 32.67 KDa
Source organism: Helicobacter pylori G27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B5Z6U8 (Residues: 1-290; Coverage: 100%)
Gene names: HPG27_536, folD
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2221
Unit cell:
a: 69.88Å b: 109.04Å c: 83.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.182
Expression system: Escherichia coli BL21(DE3)

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