› 6apv

X-ray diffraction
1.99Å resolution

Trypanosoma brucei hypoxanthine guanine phosphoribosyltransferase in complex with [(2-{[2-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)ethyl][(E)-2-phosphonoethenyl]amino}ethoxy)methyl]phosphonic acid

Released:
DOI: 10.2210/pdb6apv/pdb
PDB entry 6apv coloured by chain, front view.
PDB entry 6apv coloured by chain, side view.
PDB entry 6apv coloured by chain, top view.
Source organism: Trypanosoma brucei brucei
Primary publication:
Evaluation of the Trypanosoma brucei 6-oxopurine salvage pathway as a potential target for drug discovery.
Doleželová E, Terán D, Gahura O, Kotrbová Z, Procházková M, Keough D, Špaček P, Hocková D, Guddat L, Zíková A
OpenAccess logo PLoS Negl Trop Dis 12 e0006301 (2018)
PMID: 29481567

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine-guanine phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 216 amino acids
Theoretical weight: 24.22 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07010 (Residues: 1-210; Coverage: 100%)
Gene name: HGPRT
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand 3L4 4 x 3L4
Ligand MG 8 x MG
Ligand PEG 4 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Unit cell:
a: 89.48Å b: 94.555Å c: 109.692Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.198
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Nucleoside Hydrolase NH 36: A Vital Enzyme for the Leishmania Genus in the Development of T-Cell Epitope Cross-Protective Vaccines.
Palatnik-de-Sousa CB. (2019)
3 more