› 6awb

Electron Microscopy
6.7Å resolution

Structure of 30S ribosomal subunit and RNA polymerase complex in non-rotated state

Released:
DOI: 10.2210/pdb6awb/pdb
PDB entry 6awb coloured by chain, front view.
PDB entry 6awb coloured by chain, side view.
PDB entry 6awb coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of RNA polymerase bound to ribosomal 30S subunit.
Demo G, Rasouly A, Vasilyev N, Svetlov V, Loveland AB, Diaz-Avalos R, Grigorieff N, Nudler E, Korostelev AA
Elife 6 (2017)
PMID: 29027901
Related structures: EMD-7014

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 27-mer (preferred)
PDBe Complex ID:
PDB-CPX-215801 (preferred)
Entry contents:
25 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (26 distinct):
DNA-directed RNA polymerase subunit alpha Chains: 01, 02
Molecule details ›
Chains: 01, 02
Length: 229 amino acids
Theoretical weight: 25.34 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 6-234; Coverage: 70%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: 03
Molecule details ›
Chain: 03
Length: 1340 amino acids
Theoretical weight: 150.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8V2 (Residues: 2-1341; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: 04
Molecule details ›
Chain: 04
Length: 1369 amino acids
Theoretical weight: 151.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8T7 (Residues: 8-1376; Coverage: 97%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: 05
Molecule details ›
Chain: 05
Length: 76 amino acids
Theoretical weight: 8.65 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A800 (Residues: 2-77; Coverage: 84%)
Gene names: JW3624, b3649, rpoZ
Sequence domains: RNA polymerase Rpb6
30S ribosomal protein S1 Chain: B
Molecule details ›
Chain: B
Length: 153 amino acids
Theoretical weight: 13.31 KDa
Source organism: Escherichia coli
Small ribosomal subunit protein uS2 Chain: E
Molecule details ›
Chain: E
Length: 218 amino acids
Theoretical weight: 24.25 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V0 (Residues: 9-226; Coverage: 91%)
Gene names: JW0164, b0169, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS3 Chain: F
Molecule details ›
Chain: F
Length: 206 amino acids
Theoretical weight: 23.08 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V3 (Residues: 2-207; Coverage: 88%)
Gene names: JW3276, b3314, rpsC
Sequence domains:
Small ribosomal subunit protein uS4 Chain: G
Molecule details ›
Chain: G
Length: 205 amino acids
Theoretical weight: 23.38 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7V8 (Residues: 2-206; Coverage: 100%)
Gene names: JW3258, b3296, ramA, rpsD
Sequence domains:
Small ribosomal subunit protein uS5 Chain: H
Molecule details ›
Chain: H
Length: 157 amino acids
Theoretical weight: 16.53 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W1 (Residues: 10-166; Coverage: 94%)
Gene names: JW3265, b3303, rpsE, spc
Sequence domains:
Small ribosomal subunit protein bS6, non-modified isoform Chain: I
Molecule details ›
Chain: I
Length: 100 amino acids
Theoretical weight: 11.67 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02358 (Residues: 1-100; Coverage: 74%)
Gene names: JW4158, b4200, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS7 Chain: J
Molecule details ›
Chain: J
Length: 151 amino acids
Theoretical weight: 16.86 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02359 (Residues: 2-152; Coverage: 84%)
Gene names: JW3303, b3341, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS8 Chain: K
Molecule details ›
Chain: K
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W7 (Residues: 2-130; Coverage: 99%)
Gene names: JW3268, b3306, rpsH
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS9 Chain: L
Molecule details ›
Chain: L
Length: 127 amino acids
Theoretical weight: 14.55 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7X3 (Residues: 4-130; Coverage: 98%)
Gene names: JW3199, b3230, rpsI
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS10 Chain: M
Molecule details ›
Chain: M
Length: 98 amino acids
Theoretical weight: 11.2 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R5 (Residues: 5-102; Coverage: 95%)
Gene names: JW3283, b3321, nusE, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein uS11 Chain: N
Molecule details ›
Chain: N
Length: 116 amino acids
Theoretical weight: 12.39 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R9 (Residues: 13-128; Coverage: 90%)
Gene names: JW3259, b3297, rpsK
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chain: O
Molecule details ›
Chain: O
Length: 123 amino acids
Theoretical weight: 13.64 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 2-124; Coverage: 99%)
Gene names: JW3304, b3342, rpsL, strA
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS13 Chain: P
Molecule details ›
Chain: P
Length: 114 amino acids
Theoretical weight: 12.63 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S9 (Residues: 2-115; Coverage: 97%)
Gene names: JW3260, b3298, rpsM
Sequence domains: Ribosomal protein S13/S18
Small ribosomal subunit protein uS14 Chain: Q
Molecule details ›
Chain: Q
Length: 100 amino acids
Theoretical weight: 11.48 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG59 (Residues: 2-101; Coverage: 99%)
Gene names: JW3269, b3307, rpsN
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS15 Chain: R
Molecule details ›
Chain: R
Length: 88 amino acids
Theoretical weight: 10.16 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ4 (Residues: 2-89; Coverage: 99%)
Gene names: JW3134, b3165, rpsO, secC
Sequence domains: Ribosomal protein S15
Small ribosomal subunit protein bS16 Chain: S
Molecule details ›
Chain: S
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T3 (Residues: 1-82; Coverage: 100%)
Gene names: JW2590, b2609, rpsP
Sequence domains: Ribosomal protein S16
Small ribosomal subunit protein uS17 Chain: T
Molecule details ›
Chain: T
Length: 80 amino acids
Theoretical weight: 9.26 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG63 (Residues: 4-83; Coverage: 95%)
Gene names: JW3273, b3311, neaA, rpsQ
Sequence domains: Ribosomal protein S17
Small ribosomal subunit protein bS18 Chain: U
Molecule details ›
Chain: U
Length: 65 amino acids
Theoretical weight: 7.61 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T7 (Residues: 10-74; Coverage: 87%)
Gene names: JW4160, b4202, rpsR
Sequence domains: Ribosomal protein S18
Small ribosomal subunit protein uS19 Chain: V
Molecule details ›
Chain: V
Length: 79 amino acids
Theoretical weight: 9.06 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U3 (Residues: 3-81; Coverage: 86%)
Gene names: JW3278, b3316, rpsS
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein bS20 Chain: W
Molecule details ›
Chain: W
Length: 85 amino acids
Theoretical weight: 9.51 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U7 (Residues: 3-87; Coverage: 98%)
Gene names: JW0022, b0023, rpsT
Sequence domains: Ribosomal protein S20
Small ribosomal subunit protein bS21 Chain: X
Molecule details ›
Chain: X
Length: 65 amino acids
Theoretical weight: 7.76 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P68679 (Residues: 4-68; Coverage: 92%)
Gene names: JW3037, b3065, rpsU
Sequence domains: Ribosomal protein S21
16S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 1539 nucleotides
Theoretical weight: 498.73 KDa
Sequence domains: Bacterial small subunit ribosomal RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.7Å
Relevant EMDB volumes: EMD-7014
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Coupled Transcription-Translation in Prokaryotes: An Old Couple With New Surprises.
Irastortza-Olaziregi et al. (2020)
7 more

5 mentions without citation

Macromolecular assemblies supporting transcription-translation coupling.
Webster et al. (2021)
4 more