› 6axd

Solution NMR

Structures of REV1 UBM2 domain complex with ubiquitin and with the first small-molecule that inhibits the REV1 UBM2-ubiquitin interaction

Released:
DOI: 10.2210/pdb6axd/pdb
PDB entry 6axd coloured by chain, ensemble of 20 models, front view.
PDB entry 6axd coloured by chain, ensemble of 20 models, side view.
PDB entry 6axd coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structures of REV1 UBM2 Domain Complex with Ubiquitin and with a Small-Molecule that Inhibits the REV1 UBM2-Ubiquitin Interaction.
Vanarotti M, Grace CR, Miller DJ, Actis ML, Inoue A, Evison BJ, Vaithiyalingam S, Singh AP, McDonald ET, Fujii N
J Mol Biol 430 2857-2872 (2018)
PMID: 29864443

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair protein REV1 Chain: A
Molecule details ›
Chain: A
Length: 72 amino acids
Theoretical weight: 7.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: Q9UBZ9 (Residues: 998-1040; Coverage: 3%)
  • Best match: Q9UBZ9-3 (Residues: 234-238)
Gene names: REV1, REV1L
Sequence domains: Ubiquitin binding region

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment: 69%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

Quick links

Citations

3 review citations

Targeting protein-protein interactions in the DNA damage response pathways for cancer chemotherapy.
McPherson et al. (2021)
2 more

2 mentions without citation

Small-molecules that bind to the ubiquitin-binding motif of REV1 inhibit REV1 interaction with K164-monoubiquitinated PCNA and suppress DNA damage tolerance.
Vanarotti et al. (2018)
1 more