› 6azz

X-ray diffraction
2.4Å resolution

Crystal structure of Pfs25 in complex with the transmission blocking antibody 1190

Released:
DOI: 10.2210/pdb6azz/pdb
PDB entry 6azz coloured by chain, front view.
PDB entry 6azz coloured by chain, side view.
PDB entry 6azz coloured by chain, top view.
Source organisms:
Primary publication:
Molecular definition of multiple sites of antibody inhibition of malaria transmission-blocking vaccine antigen Pfs25.
Scally SW, McLeod B, Bosch A, Miura K, Liang Q, Carroll S, Reponen S, Nguyen N, Giladi E, Rämisch S, Yusibov V, Bradley A, Lemiale F, Schief WR, Emerling D, Kellam P, King CR, Julien JP
Nat Commun 8 1568 (2017)
PMID: 29146922

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-230991 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
1190 antibody, heavy chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 223 amino acids
Theoretical weight: 23.99 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
1190 antibody, light chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 215 amino acids
Theoretical weight: 23.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Pfs25 Chains: A, D
Molecule details ›
Chains: A, D
Length: 183 amino acids
Theoretical weight: 20.1 KDa
Source organism: Plasmodium falciparum
Expression system: Homo sapiens
Structure domains: Plasmodium vivax P25 domain

Ligands and Environments

2 bound ligands:
Ligand ACT 6 x ACT
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 53.572Å b: 203.136Å c: 65.486Å
α: 90° β: 109.89° γ: 90°
R-values:
R R work R free
0.192 0.191 0.217
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Malaria Vaccines: Recent Advances and New Horizons.
Draper et al. (2018)
6 more

2 mentions without citation

Molecular definition of multiple sites of antibody inhibition of malaria transmission-blocking vaccine antigen Pfs25.
Scally et al. (2017)
1 more